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| Speeding rabbit morula embryo freezing and research to determine viability
970 [5] BankH & MaurerRR: Survivaloffrozenrabbitembryos. ExplCellRes89: 188. t974r6] RenardJPetaI: Two-Stepfreezingoftwo-eel/rabbitembryosterpartialdc-hydrationatroomtemperature. JRep-rodFert71: 578. t984 [7] Mi3amotoH & IshibashiT: Liquidnitrogenvapourfreezingofixtouseembryos. JRe-prodFert78: 47l, t9868j Wang Yanrong , Liu Bin : rabbit embryo cryopreservation, Beijing University of hairpin 20 (2)  29,Tory Burch Outlet,1988 [9] LeiboSP & MazurP: MethodsforthepreservationofmammalianembryosbyfreezingInMethodsinMammalianReproduction (DanielJC.ed.) Pl79. Academicpress, NewYork, 1978 [10] WhittinghamDO: Vlabilityassaysformammalianova. Cryobiology15: 245,mbt shoes sale, 】 978 (I989 Si Received on May 15, l1 ) StudybyUltrarapidFreezingMethodandEvaluationofViabflityofRabbitMorulaeWangYan-rongLiuBin (DepartmentofHistologyandEmbryology, BeqtngMedicalUniverdty) ABSTRACTThiscxperimentanalysestheeffectsofdifferentconcentrationsofcryoprotectantglycerolandSLIcr0, ~ diluentontheviabilityofrabbitmorulaeduringultmrapidfre-ezingandthawing. Whentheconeentrationofsucrosediluentwas1.0M. andthatofcryoprotectant3.0Mor4.0MglyceroI +0.25 MSUCTOSC. theeryoprotectfvceffectisdistinctlygood. Thedevelopmentrateofthawedembryosinvitrowas73.68and80.56,mbt zapatos, re ~ ixetivcly. Thcbirthratewas13.33, similartothatofthecontrolgroup (n.1l). FluorescencetestsshowthatFDAandDAPItestsaretherapidandprecisemothodsforevaluationoftheviabilityofthawedembryos. Theeffectontheultras. tructureofembryosfollowingfreezingandthawingwasobserved, andtheresultshowsthathigherglyceroleoncentmtionCOUldeffectivelyprcservetheuhrastruetureofembryos. Keywords: I. Yltrarapidfreezing,Mbt Schuhe Günstig, Rabbitembryo, Glycerol ~ sucrose, Ultrutructure
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