 Wysłany: Czw 23:59, 03 Mar 2011 Temat postu: Cytochrome P4501A1 gene mutation and lung cancer s |
|
| Cytochrome P4501A1 gene mutation and lung cancer susceptibility
Ling, Gao Yang, Zhang bridge. Cancer, 1998,18:269 LeeE, ZhaoB, Moochhala, eta1. Phatmaengen ~ ics, l994r4: 355HayashiSlrWatanabeJ, KawajiriK. Tokyo: Pro-ceedingsofJapaneseBiochemicalSocietyr64thAnnualMeeting, Tokyorl991r997ZhangZYrFascoMJrHuengLreta1. CancerRgs, l996, 56:39269 CroftsF, TaioliE, TrachmallJ, etalCarcinogcnesis, 1994,15:296 l20CosmaG, CroilsF, TaiOliE, eta1. JToxicolEnvffonHealth, l993, 4:30921 PcterscnDD, MckinneyCErlkeyaK, a1. AmJHumGenet, l99l, 48:72022 LandiMT,UGG boots Italia, Be ~ azziPA, ShieldsPG, eta1. Phmmacogencs, l994, 4:24223 JacquetM, Lambe ~ V, BaudottxE,keen shoes sale, spit a1. ElitJCancer, l996, 32A: l70l24MooneyLA, Bel1DA, SantellaRM, eta1. Carcinogenesis, 1997, l8: 50325WangY, lchibaM, IyadomiM,mbt shoes italia, a1. IndHealthrI998, 36:33726 RojasM, AlexandrovK, CascorbiI, eta1. Pharmaengene0cs, 1998,8: l0927SchoketB, PhillipsDH, KOStiCS, eta1. Clrcinogcncsis, l998r19: 84128Kawaj ~ K, EguchiH, NakachiK, eta1. CancerRcs, l996,ugg schuhe, 56:7229 Prg0dddRMtBennettWP, GuinenDGreta1. Phmanac ~ geneties, 1998r8: 50330DcnissenkoMF, PsoArTangM-S, a1. Science, l996, 274:430 high-throughput analysis of gene expression in a comprehensive understanding of technologies like gene expression in tissues and organs of a field spectrum, need for fast, parallel to the screen as much as possible (preferably all) of the gene samples. Ma Xi-German Institute of Molecular Genetics Cahil1, D, who mediated in the shake drop ship board (microtitreplate) on high-throughput analysis of gene expression spectrum of technology fields: a large number of cDNA library clones were dot matrix board in the shake drop , operating fully automated, that is, a service developed a robot control system, 3 axis jjl Nang system integration department. Installed planted with 384 needles, resolving power of 5, it can point per 300 points c, so that in the conventional 22x22cm nylon membrane can be ten points on the 5.5 million, making it the high-density point barrier. The system also increases the sampling rate, to 3900 clones / hour. Board can be in the shake drop parallel PCR reactions (simultaneous amplification of the first ten cDSA clone 51840), it reached a high throughput of this analysis. The authors also developed another method, namely the use of the known nucleotide oligonucleotide fingerprinting extension field spectrum ONF, Oligonuclootidefingerprinti Ⅱ R) identification cD library. As follows: a group of 200 ten and being widowed Wu nucleotide point barrier lying in the membrane of the cD hybridization, the hybridization of each cDNA clone have ten field spectral fingerprints of DNA sequences, similar to those fingerprints combined into a group or nest of , after a blind according to DN. , T deposited sequence database built from the theoretical fingerprint comparison, you can know these are known genes or a new base circle, if a known base! Zl cut off the feet can be further know how genes. On the above principle is also special for protein analysis. (Fang Fude combination of genes taken from the meeting of 99 international human Collected Works,mbt scarpe outlet, 1999, Australia) |
|
