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Detection of DNA amplification with the sickle cell gene


7:9 B,. Saiki, K. pill Cts1. l986. . Mature. , 2;: L63 ¨ m ... genetic paternity of l6 volume DetectionofSickleC. IiQeaebAadysisol & ~ plifiatDNASequencesHuangShuzhenShengMinZhaodiuqiao. iuXiaokunZengYkao (Laboratory. | MedicalGenaie ~. ShangkaiChildren'sHoJpha1. Shanghm) Wang. isong,} (Issth ~ tt. fGe ~ dcsF.dauUMverdty ~ Shanghai) HeMingxiZhuJimianLiuWeipeiLiWeiwentTheFirslPeople'H. Id. | Fu ~ kan. Guangdong1ABSTRACYrThispaperdescribesatechniqueofDNAamplificationinvitroanditsapplicationondetectionofsicklecellfHbSgene. GenomicDNAwasmicroextractedfromdrledbloodspecimenofthefirstpatientwithsicklecelttraitinChina. TargetDNAsequencewasanapli-fledbythepolymerasechainreaction (PCR) withtheprimers8if5 a AcAcAAcTCTGTT-CACTAGC, -3 ') andB | ('-cAACTTCATCCACGTTCACC-3 ') thatprimedamplificationofaD110 a base-pair (bp) segmentof8globingeneTamplifiedDNAwasdigestedwith4restrictionendonucleaseMs £ I】,discount cosmetics mac, whichhasarecegnitionsiteatcodon6inthenorma1globingene, andcleavedthenormalamplifiedBglobinDNAintotwofragmentsof54bpand56bpwhichwasasanoverlapbandinagarosegelelectrophoresis, whilethell0bpfragmentam-plifiedfromDNAofsicklecellmutationrema/neduncleavedowingtoasinglebasesubstitu-tion (AT) atcodon6inthemutationDNAamplificationmethodisrapid,mbt shoes on sale, sensitiveandsimple, anddoesnotrequireradioac-tireprobes. Besides, thePCRamplificationc & nbecarriedoutontheDNAextractedfromdriedbloodsamplesSothetechniqueisveryusefulforgenediagnosisandcarterscreeningofgeneticdiseaseKeywords: ChineseHb8gene,laguna beach jeans men, DNAamplification, PolymerasechainreactionRecf Ⅵ dofJu4.1988 'Thinworkitpardysupl ~ rtedUSpFI $ Re. catchGratF1LB a 29625.
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