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Asparagus officinalis pollen in vitro and plant regeneration

Education in the process of chromosome doubling occurred at some stage or other chromosomal aberrations, leading to complex plants produce r, especially in times of callus phase of unstable chromosomes,ugg boots italia, chromosome doubling, and other prone to chromosomal variation. We also observed the following characteristics. (1) The variation range of chromosomes, extensive coverage. Observed from the I \; triploid cells accounted for 1.88; four times and four times more cells in times of 16.88; aneuploid cells in 0.63. (2) pollen callus high frequency of chromosomal aberrations. Callus with a secondary examination, the interval of 25 days, and the variation in Table 2. Fusarium head two blocks of 2 bad a group of children injured in the times of dam block variation TaMe2Plmdymutatiomconditiononthe5ca ¨ uBundertwomier ~ eopecheek10gO a 11 - 091g91-01-25 Single piece two pieces of autograft haploid diploid polyploid times No. HaploidsDipoidsPolyploidsHaplosDip [oidsPolypl ~ dsNo. Cell argon teach a hundred small boats roar thing to teach small cell spore cell teach teach teach Noof percentage of base fine roar No. what percentage of N0. ofNoof rate per boat No. Chapter percentage 0f No. 0 {Chapter ()()')()()() eelIct1lcc ¨ eelIc percentage】 1ceItA77523B733.33142g527.78844.44527.7BA288. Pina l5652226.091g73.08726. g2Al25l5631856.2528.132605-1g61.20322A15l15476. g23ll_54l3452172.41774.14A1041473-68526.327B34.7B Total 35a2.386551.612l16011575572037Total can be seen at 3478; pollen callus after 75 days of culture, the proportion of haploid cells decreased by 18.76, the proportion of diploid cells increased by 5.59, an increase of 13.17 polyploid cells. This shows that haploid tissues are more likely than chromosome doubling of diploid tissues. (3) pollen callus high percentage of polyploid cells. Anther culture in solid medium,asics running shoes, the callus induced differentiation only polyploid cells in total cell number of 1.8, while the pollen produced by callus culture of polyploid cells in 18 or so. 3 Discussion 3.1 The anther wall in pollen development role Sunderlancl pointed out long ago: pollen development and anther wall into a small plant related to the role. Our experiments show that the pollen culture head wind and Britain 4: asparagus pollen in vitro and plant regeneration 269 support,UGG Boots Outlet, the pollen must be more than 7 days pre-training, can go to differentiation and to further develop into cell clusters and eventually form a callus. Some researchers use conditioned medium (medium supplemented with anther culture medium) or in medium with drug residue wall tissue extracts have not been successful. Although we also added in the medium a certain amount of active material, pre-training is still required. The anther wall tissue also provides other, more important metabolites. As in the rice anther Zhonghua Xin and other soluble protein and starch enzyme electrophoresis analysis pointed out that pollen to anther wall differentiation in addition to the absorption of storage into play the role of the metabolic library, but it seems there is some protection and control role. 3.2 The plant hormones role in pollen development of plant hormones is the role for pollen development and the complexity of the different pollen need to start hormone differentiated type, concentration and ratio of each of its specificity. This experiment uses o. 5ppmNAA only in the MS medium to induce callus. This shows that a hormone in the role of different media is inconsistent. Authors suggest that exogenous hormones on the development of asparagus pollen is necessary. The experimental results show that: 2,4 ~ D,ecco golf shoes, 6BA and NAA used in conjunction with the promotion of pollen to a greater differentiation. Pollen alone 0.5ppm/LNAA to the promotion of differentiation are less effective. But higher than in 2,4 D,p90x workout schedule, 6BA cell mass under the conditions of existence of the degradation of disintegration also weak. Pollen starts to divide and develop into callus cells two stages of the requirements of different hormones. On the plant hormone