Yu Ye Tang on adipose cytokines _1002 -

e054831577

,[link widoczny dla zalogowanych]
Yu Ye Tang on adipose cytokines

drochlorideinLianzhenpil1. MethodsTheHPLCconditionswereasfoHows: C18Column (200mmx4.6mm, 5m), columntemperature20 ℃, acetonitrice: 0.2% sphoricacidwater (25:75) asmobilephase,[link widoczny dla zalogowanych], theflowratewas1.0m1/min. andthedetectionwavelenthwasat345nm. ResultsThecalibrationcuiNeofberberinehydrochloridewas: Y = 42148043.09245X a 243222.939726. r = 0.9991 (n = 6). Thecontentofberbefinehydrochlorideshowedagoodlinearrelationshipintherangeof0.00988 ~ 0.0988mg/m1. Theav-eragerecoverywas100.68%, andRSDwas1.18%. ConclusionThemethodisaccurate. reliable. andcanbeusedforqualitycontrolofLianzhenpil1. Keywords: LanzhenPill; Berberinehydrochlofide; HPLC: Qualitystandard with Zhen Pills by berberine, Ligustrum lucidum composed of compound for the treatment of advanced cancer chemotherapy-induced renal failure. In order to improve bioavailability, to facilitate quick, especially for the development of pills. To ensure the quality of even the Ching pills,[link widoczny dla zalogowanych], the active ingredient in this experiment berberine as an index of berberine hydrochloride by high performance liquid chromatography to determine the content of their. Reported below. 1.1 1 reagent and instrument reagent Coptis, Ligustrum lucidum (purchased from Shijiazhuang lerentang, was identified consistent with the 2005 edition of Berberine hydrochloride reference substance (China Pharmaceutical and Biological Products, batch number 110713-200208); acetonitrile (HPLC grade, Tianjin Concord Technology Co., Ltd., batch number 051107); methanol (HPLC grade, Germany FisherScintific, Lot 061,495), other reagents used in the experiments were of analytical grade. Received date: 2007 ~ 7-26; revision date :2007-10-26 Author: Lee Chun-hua (1965 A), female (Han), Hebei Lixian people,[link widoczny dla zalogowanych], currently professor of Hebei Medical University, a master's degree, mainly in traditional Chinese medicine pharmaceutical research. 1.2 Instrument performance liquid chromatograph Shimadzu SPD a 10D; detector (SHIMADZU, SPD a 10P); N2010 chromatography workstation; electric DHG a 9070A-type blast oven (Shanghai a box of Scientific Instruments Co., Ltd.); Analysis Balance (SartoriusCP225D); vacuum pump (DP a 01VACUUMPRESSUREPUMP). 2 Methods and Results 2.1 Dumas Company DiamonsilODSC column chromatographic conditions (4,6 mmx200mm, 5m); mobile phase of acetonitrile: 0.2% phosphoric acid water (25:75); detection wavelength 345nm; column temperature 20 ℃; flow rate of 1 .0m1/min; injection volume 20l. 2.2 Preparation of reference substance solutions that take 105% of the dry precision of berberine hydrochloride reference substance 5h 9.88mg, set 100ml flask, add hydrochloric acid: methanol (1:100) 5min ultrasonic treatment to dissolve and set the volume scale, that is, too (0.0988mr/m1). 2.3 Preparation of the test solution with Chen taking 40 pills,[link widoczny dla zalogowanych], small study, the check amount, said precision set 1.47981g, set 100ml flask, add hydrochloric acid: methanol (1:100) amount, ultrasonic treatment 20min ( 25%), and acid: methanol (1:100) to scale, shake, filter membrane with 0.45m, take continued filtrate as the test solution. * 1451 *

				fora.pl - załóż własne forum dyskusyjne za darmo Powered by phpBB © 2001, 2002 phpBB Group Avalanche style by What Is Real © 2004