Virus cultivation, determination of total alkaloid -

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Virus cultivation, determination of total alkaloids from Pinellia wild places

Motherwort,[link widoczny dla zalogowanych], Angelica, red peony root, red sage root, turmeric, Achyranthes,[link widoczny dla zalogowanych], Citrus aurantium, woody composition of 11 herbs, with blood circulation of qi,[link widoczny dla zalogowanych], stop bleeding menstruation function. The symptoms of menstruation due to stagnation of qi excessive menstruation. Years of clinical application showed that the product is effective, no adverse reactions. Motherwort is at Fangzhong Jun drugs, mainly containing Stachydrine, Leonurine, Motherwort be active ingredients and other alkaloids. Determination on Leonurus reported in the literature have acid-base titration, the total alkaloid acid dye colorimetry, TLC, HPLC method to measure Stachydrine hydrochloride, basis, after repeated tests, to determine the cation exchange column by HPLC, determination of preparation Stachydrine hydrochloride content, the results show that the method is more accurate, sensitive, reproducible and easy to operate. 1 1 instrument and reagent .1 a 10AT instrument LC liquid chromatograph pump type host (Shimadzu), SPD-IOA UV detector (Shimadzu); N-2000 chromatography workstation (Zhejiang University Zhida); AD a 2 parts per million an electronic balance (U.S. PE company); PHS-25 pH meter (Shanghai Lei magnetic). 1.2 trial Stachydrine hydrochloride (Pharmaceutical and Biological Products, Lot :0720 -9807, for the determination of use) ; other chemical reagents were of analytical grade. for the test sample: the Company's pilot production and small scale samples. 2 Stachydrine hydrochloride by high performance liquid chromatography and methods of inspection 2.1 Preparation of standard solutions, accurately weighed Stachydrine hydrochloride reference substance 2mg, home 5lnl volumetric flask, add 0.1tool · L sodium dihydrogen phosphate to the scale, ultrasound allows the dissolved, shake, that was (per 1ml containing hydrochloric acid Stachydrine 0.4nag). 2.2 Preparation of test solution to remove the goods small study, of about 5.0g, accurately weighed, add alkaloids; In addition, the alkaloid content can represent the quality need Pinellia experiments, because clinical Preliminary experiments show that the species was no significant difference in efficacy. For total alkaloids in Rhizoma determination, the literature has reported a number of ways. If the weight of law J, titration, HPLC and so on. Among them,[link widoczny dla zalogowanych], the weight method is cumbersome, large amount of samples, reproducibility poor. titration method is also easy to extract the introduction of repeated errors; high performance liquid chromatography, the weak UV absorption of ephedrine hydrochloride, a trace amount of alkaloids in Rhizoma makes it difficult determination. the present study, acid dye colorimetry, according to a certain pH medium, alkaloids and hydrogen ions to form salts, a number of acid dyes in this condition can be combined with the salt ions into the colored complex quantitative , were some of the principles of organic solvent extraction of quantitative; ephedrine hydrochloride as a reference material in the pH = 5.40 under the conditions of quantitative and bromothymol blue color, the alkaloids in Rhizoma down to earth in this condition can and the color of the bromothymol blue can be measured out to obtain the relative content of total alkaloids. The method is sensitive, the results are stable, easy to operate, is the determination of total alkaloids from Pinellia a better way . In this experiment,[link widoczny dla zalogowanych], carried out on the same samples were ice cold ultrasonic extraction and ultrasonic extraction at room temperature comparison of experimental results show that ice cold ultrasonic extraction percentage measured value is stable and safe during the extraction process , so choose low-temperature ultrasonic samples.

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