|
 |
Wto 0:26, 18 Sty 2011 |
 |
Autor |
Wiadomość |
06nike7394ud
95%

Dołączył: 26 Paź 2010
Posty: 796
Przeczytał: 0 tematów
Ostrzeżeń: 0/5 Skąd: England
|
Temat postu: tory burch outlet Detection of DNA amplification w |
|
|
,[link widoczny dla zalogowanych]
Detection of DNA amplification with the sickle cell gene
7:9 B,. Saiki, K. pill Cts1. l986. . Mature. , 2;: L63 ¨ m ... genetic paternity of l6 volume DetectionofSickleC. IiQeaebAadysisol & ~ plifiatDNASequencesHuangShuzhenShengMinZhaodiuqiao. iuXiaokunZengYkao (Laboratory. | MedicalGenaie ~. ShangkaiChildren'sHoJpha1. Shanghm) Wang. isong,} (Issth ~ tt. fGe ~ dcsF.dauUMverdty ~ Shanghai) HeMingxiZhuJimianLiuWeipeiLiWeiwentTheFirslPeople'H. Id. | Fu ~ kan. Guangdong1ABSTRACYrThispaperdescribesatechniqueofDNAamplificationinvitroanditsapplicationondetectionofsicklecellfHbSgene. GenomicDNAwasmicroextractedfromdrledbloodspecimenofthefirstpatientwithsicklecelttraitinChina. TargetDNAsequencewasanapli-fledbythepolymerasechainreaction (PCR) withtheprimers8if5 a AcAcAAcTCTGTT-CACTAGC, -3 ') andB | ('-cAACTTCATCCACGTTCACC-3 ') thatprimedamplificationofaD110 a base-pair (bp) segmentof8globingeneTamplifiedDNAwasdigestedwith4restrictionendonucleaseMs £ I】,[link widoczny dla zalogowanych], whichhasarecegnitionsiteatcodon6inthenorma1globingene, andcleavedthenormalamplifiedBglobinDNAintotwofragmentsof54bpand56bpwhichwasasanoverlapbandinagarosegelelectrophoresis, whilethell0bpfragmentam-plifiedfromDNAofsicklecellmutationrema/neduncleavedowingtoasinglebasesubstitu-tion (AT) atcodon6inthemutationDNAamplificationmethodisrapid,[link widoczny dla zalogowanych], sensitiveandsimple, anddoesnotrequireradioac-tireprobes. Besides, thePCRamplificationc & nbecarriedoutontheDNAextractedfromdriedbloodsamplesSothetechniqueisveryusefulforgenediagnosisandcarterscreeningofgeneticdiseaseKeywords: ChineseHb8gene,[link widoczny dla zalogowanych], DNAamplification, PolymerasechainreactionRecf Ⅵ dofJu4.1988 'Thinworkitpardysupl ~ rtedUSpFI $ Re. catchGratF1LB a 29625.
More articles related to topics:
tory burch outlet Branch of the Chinese clothing c
buy ghd hair Foliar application of phosphorus, pot
Tory Burch Shoes ESSENTIALLY NORMAL + SMALL COMPAC
Post został pochwalony 0 razy
|
|
|
|
|
|
|
 |
 |
|
 |
|
 |
Możesz pisać nowe tematy Możesz odpowiadać w tematach Nie możesz zmieniać swoich postów Nie możesz usuwać swoich postów Nie możesz głosować w ankietach
|
|
|
|
|
|
|
fora.pl - załóż własne forum dyskusyjne za darmo
Powered by phpBB © 2001, 2002 phpBB Group
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|