Patch clamp technology lecture series the third pa -

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Patch clamp technology lecture series: the third part of the patch clamp technique biopsy

5 [stuanc, Jts coffee Ⅱ Aeti ~ propagationor D0dtH-V. $ AkmannBPatchclampnjinfromil ... m, q1dr1 ... f ... a sinbraindicesusinginfraredmicroscopy. PflefsArch. 1 Shuai 3; 42s: 51l ~ 【8 [11] TakahashiTIntrace] lularrecordingfr ~ n, visuaTlyident, ti ~. J ¨ l ∽ Ⅲ in ¨ tI1cordslicesPRoyrLondonB. 1978; 202:417 ~ 421:12] Takahm ~ hiTMembran ~ c-ntsmvisuallyidenti ~ 'ied a J ~. ... 1 f4 pages) on the membrane fluidity quantitative and qualitative analysis. fluorescent membrane probe excited by polarized light, the emitted light depends on the polarity of the rotation of fluorescent molecules, which depends on the degree of freedom and orderly movement of fluorescent molecules around the membrane fluidity, thus indirectly reflects the polarity of membrane fluidity measurement. This determination of membrane fluidity in the membrane of phosphatidic acid composition analysis, drug effects and action sites, the temperature response measurement and comparison of species has an important role. on adherent cells were isolated and screened for anchorage dependent cells. This does not change the cell culture medium surrounding environment,[link widoczny dla zalogowanych], degree of cell spreading and growth of the state of separation methods is essential. First, adherent cells cultured in a special dish, and then For high-energy laser with selected cells in the octagonal geometry around the cut, not selected due to the octagonal-shaped cells were removed in addition, the choice of cells and morphological analysis of fluorescence dependent on specific parameters. The separation methods in particular for selected small number of such mutant cells, metastatic cells, and hybridoma cells, even one in a million chance of the cells is also very satisfactory. Another way is to use high-energy laser automatically without killing cells, leaving intact living cells subgroup continued to train,[link widoczny dla zalogowanych], the method is also based on cell morphology and fluorescent properties, this method is particularly suitable for larger number of cells on the choice of the above two methods. You can scan the whole cell population; based on physical and biochemical characteristics of cell sorting; on one in a million chance of occurring mutant cell screening; does not change the cell shape, type of activity and the cloning of cells; separation of cell subsets for quantitative fluorescence analysis; automatically stored cell location to determine the specific duplication of cells. has been based on lateral movement of membrane proteins, gap junctions and transfection characteristics of the screening for mutations in cells; isolation and cloning, such as smooth muscle cells, Chinese hamster ovary cells and other stem cells into human teratoma cell lines: isolation of human T cell populations. black melanocytes, hepatocytes and other cell subsets, showing high efficiency and precision of the laser can also be used as a chromosome cutting, removal of a series of processes in the neuron cell surgery. can also be used to move the operation of light traps small particles and cell structure, the new technology widely used in chromosome movement, movement of organelles and cytoskeletal elasticity measurement experiment. using laser cytometry and flow cytometry, can be studied in greater depth the characteristics of a xiao cells. and the isolation and cloning of specific cells. further cancer research, cell biology,[link widoczny dla zalogowanych], cytoskeleton, developmental biology, embryology,[link widoczny dla zalogowanych], genetics, immunology, anatomy, in situ hybridization, chromosome, neurobiology, pathology, pharmacology, multidisciplinary, multifaceted, comprehensive and effective multi-angle studies. further elucidate the mechanism of cells, explaining various biological phenomena, improve the academic research level, and ultimately improve the level of diagnosis and treatment of disease. Li Yan (-il ~ Department of Biology,[link widoczny dla zalogowanych], Beijing Normal University)

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