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AntimicrobAgentsChemother. 1992,364844855 l_ewisK. MuhidrugresistancepumpsinbacteriavariationsonathemeTreadsBiochemSci, I99419I1101235MipauchiS, KomatsubaraM, KamoNInarchaebacter】 a,[link widoczny dla zalogowanych], thereisadoxorubicineffluxpumpsimilartomammalianPglycoprorein. BiochemBiophysActa. 1992.111OI44 a I50.7BronSPlasmids. In: HarwoodCRCuttingSMed. blolecularbioLogicalmethodsforBacillusChichcsler, E: ngland: fog mouth WilevS0ns. 1gg0,[link widoczny dla zalogowanych], 40143.8ChangS, CohenSNHighfrequencytronslo: t ~ PonofB ~ uhtih'sprotopla ~ tsbypIasmidDNA. blolGenC-enet, 1979,168; 111-1159AnagnoztopoulosCtSpizizeaJRequirementsfortransformationinBaxillussubtilis. JBacterio1.1961, 8774174610SambrookJ, FritschEFtManiatisTMoLecularcLoning: alab-oratorymanua1.2nded. NewYork: ColdSpringHarborLabD_ratoryPresst198911NikaidoH-Preventionofdrug ~ ccesstobacterialtargetspermeabilitybarriersandactiveeffLux. Science, 1994.264382. 8.12 Tang Xin Yun mad nonspecific bacterial resistance to multiple drugs Microbiology. 1998.95 (2) :107-110. (Received: 19980223 Revised :1998-0608) PCR rapid detection of Shigella in feces and invasive Escherichia coli gene and its clinical application IpaH Ran Ding Jianqiang line Mayi Lin Ya spine Kong boiling quickly from the stool specimens were extracted bacteria DNA as the polymerase chain reaction (PCR) template, 1 self-designed pair of specific primers bit Yu Zhi Shigella and invasive E. coli plasmid DNA on the chromosome and invasion plasmid antigen multi-copy (Ipa) H gene amplified product was 620bp, often of the unit = 310003 Hangzhou. Zhejiang Medical University, a courtyard of a building with strong chip (now Affiliated Sir Run Run Shaw Hospital, Zhejiang University) - Mayi Lin,[link widoczny dla zalogowanych], Ya-Gang]: Infectious Diseases Hospital, Hangzhou, Hangzhou Ran coffin f) Regulation of bacterial culture detection rate of 20 common control with intestinal standard strain of pathogenic bacteria to extract DNA PCR reaction PCR reaction on the way to test the specificity of DNA sequence analysis using the method of PCR amplification products of the specificity of the detection test = 8l were patient stool samples positive for bacterial culture 27 cases of Shigella bacteria 25 to 2 strains of Shigella within + culture positive rate was 33.3, PCR detected 39 positive cases, the positive rate was 48.1, PCR detection of bacterial culture positive rate than conventional The positive rate of 14.8,[link widoczny dla zalogowanych], including bacterial culture positive specimens were positive for PCR test,[link widoczny dla zalogowanych], 29 cases of patients after l week after formal treatment, PCR detection of l2 down still positive. Statistical analysis showed there was significant difference between sex (P <0.05). Standard PCR detection of bacteria and DNA sequencing of PCR products showed that we designed PCR method has high specificity. PCR used in this study is a quick and easy, high specificity and sensitivity of the dysentery pathogen detection strong modern molecular biology methods. (Received; 199810-16 Revised: 1999c308)
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