Ictalurus Xenocypris antigen of Aeromonas and indi -

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Ictalurus Xenocypris antigen of Aeromonas observation and indirect vaccination effects of ELISA test

Reproductive morbidity and mortality observed 1.10ELISA random testing pool from the pilot and the control of the selection pool l2 fish. Cut pericardial cavity with blood samples. BCG syringe directly with blood. After the blood refrigerator at 4 ℃, put out after 20min centrifugation, 3000r/min. 15min after the draw to the EP tube top backup serum, respectively,[link widoczny dla zalogowanych], the test group and control serum were added each l2-coated plates, 100ul / hole,[link widoczny dla zalogowanych], after dilution, HRP antigen after the termination of the color steps. OD values measured with a microplate reader and record analysis. 2 Results 2.1 Experimental group of mice all survived after virus attack, the control group all died. 2.2 vaccine proved effective in the protection of human infection after the primary immunization 50d, 70d, 90d, respectively, to do artificial infection protection test. Results were not immunized fish compared to the control group decreased mortality rate 44.5% (50d), 50% (70d),[link widoczny dla zalogowanych], and 40% (90d), see Table l. Experimental infection of the above mentioned 3 deaths in each group two fish liver. For blood smear staining and nutrient agar, nutrient agar and MacConkey agar common infection bacteria test done. Staining results were found and a large number of separation and recovery to the original infection of bacteria 2.3ELISA Junichi testing after the initial immunization results in the 50d, 70d, 90d,[link widoczny dla zalogowanych], respectively,[link widoczny dla zalogowanych], do ELISA testing, the results showed that the experimental group was immune OD value than fish OD values of the control group were much larger. And experimental group the number of fish significantly increased serum antibody, antibody production and in accordance with law. And immune antibody relatively higher after 70d. Table 2.3 discussed in this study. Inactivated with formaldehyde, Aeromonas hydrophila (AerolTl ~ no_8hydrochila) immunogen inoculation Xenocypris health Ictalurus. After a certain time to do artificial infection test and ELISA testing. Tests show that the fish on Aeromonas hydrophila can produce a good immune response. Its protective effects and antibody levels have some correlation. These results. Xenocypris further Ictalurus Aeromonas infection of the specific immunization provided a theoretical data in the research. Sun Jian, and so fl995 ') [5] have reported with Aeromonas hydrophila vaccine preparation of the whole immune carp induced immunity. After immunization with the homologous bacterial attack has proven to have some protection. At the same time. Test ways to prove a direct impact on the immune vaccination effect of direct injection is superior to immersion. He road from the results in Table 1 Table 2ELISA artificial infection test results MULTISKANEXPRIMARYEI. 2.3DUALWELENGTHMODECONTINUOUOVEMENT1. FILTER4502. FILTER630MIXINGTIME2FASTDELTAABSORBANCES Note: Table 2A row, C row, E row of 12 holes were the experimental group was immune fish 50d, 70d, 90d after the test results; B Pai, D Pai, F row of 12 wells were the control group of fish was 50d, 70d, 90d after the test results; G-H blank row of 12 holes holes, respectively. f19971 [6] reported that with Aeromonas hydrophila vaccine preparation of the whole to add a fee's incomplete adjuvant vaccine inoculation silver carp. Show that adding adjuvant has increased the ability of the immune response of fish these findings. Of fish Aeromonas hydrophila provides a scientific basis for immunization. This paper describes only the Aeromonas hydrophila Ictalurus Xenocypris on the immune effects observed. Therefore, this method will continue to be. Research and experiment with different types of Aeromonas Ictalurus immune problems of interaction Xenocypris Received Et A O1-06 of 12008

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