Blood emulsion on diabetic mice in liver and muscl -

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Blood emulsion on diabetic mice in liver and muscle glycogen synthase kinase expression

No. 7 ratio (C),[link widoczny dla zalogowanych], extraction times (D) 4 factors, 3 levels of each factor (the specific experimental factors and levels shown in Table 1), with L. (3) orthogonal experimental design, choose the best extraction conditions. Table 1 Factors of the results in Table 3 level single factor 3.1 3.1.1 Ultrasound Effect of time on the extraction of single-factor test based on ultrasound,[link widoczny dla zalogowanych], measured in parallel under the conditions worked out 3 times the extraction time on the organic acid content. With the increase of extraction time,[link widoczny dla zalogowanych], organic acid content is also increasing, 25min after the growth slowed down, so choose the orthogonal 20min or so. 3.1.2 Ultrasound Effect of temperature on the extraction of single-factor test based on ultrasound, measured in parallel under the conditions worked out 3 times extraction temperature on organic acid content. As the extraction temperature, the organic acid content also increased, 45 ℃ after the growth slowed down, so choose the orthogonal experiment is about 35 ℃. 3.2 Orthogonal experimental design based on single factors, in order to investigate the interaction parameters of various conditions, experiments designed orthogonal four factors and three levels to determine the application of L. (3) orthogonal experimental design. The results shown in Table 1, Table 2. Factors in the table A (time) of its three levels corresponding to the extraction percentage of the value of 2 maximum level, so take the best of its level 2; factor B (temperature) to the level of the value of 3 maximum; factor C (solid-liquid ratio) to the level of the value of 2 maximum; factor D (the number of extraction) to the level of the value of 3 maximum. The results showed that the optimum extraction conditions of organic acids AB. CD, the ultrasonic time of 20min, ultrasonic temperature 45 ℃, liquid ratio 1:10, extract 3 times. According to variance analysis of factors affect the size of the extraction of organic acids: the number of> time> temperature> solid-liquid ratio. The extraction process is the extraction of the largest number of factors,[link widoczny dla zalogowanych], influencing factors are the least liquid ratio. 3.3 promote the growth of casual verification experiment to take 10. O0g, solvent 100mL. According to the optimal extraction conditions screened A. B. C. D. Parallel to the extraction of 3 times the content of organic acids were measured. The results in Table 3. Table 2L. (3) orthogonal experimental design and proven results, the optimum extraction conditions based on the measured content of organic acid content of greater than the orthogonal design. 4 Conclusion and promote the growth of water-soluble components of the best powder in the extraction conditions: 20min ultrasonic ultrasonic time temperature 45 ℃, liquid ratio 1:10, extract 3 times. Promote the growth comes mainly from scattered group of water-soluble components in the prescription of the hawthorn, licorice, Burnet, etc., so the present study,[link widoczny dla zalogowanych], organic acids evaluation method is simple, reasonable and feasible.

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