_3544 Tissue culture and rapid propagation of Gerb -

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Tissue culture and rapid propagation of Gerbera

I, IAA; BA10 + KT10 + NAA0.2mg/I1 treatments. Each treatment by adding 0.6 and 3 sucrose agar. ② proliferation medium: BA2mg / I + NAA0.2mg / I +0.69 / 5 agar +39 / 5 sucrose. ③ rooting medium: 0.05mg/IIBA +0.6 agar +39 / 5 sucrose. Culture conditions: temperature (23 ± 2) c, illumination 15O0 ~ 2000Ix, light, asked 12h / d. [Received Date] 2002-08 A l2 [Author] Huang Yanfen (1967 a),[link widoczny dla zalogowanych], female. Research assistant. In plant tissue culture work. * Key issues in Guizhou Academy of Agricultural Sciences, Thanks to Xiong Yuan, Sun Ruifeng gay support, grateful j No. 6 Huang Yanfen other tissue culture propagation of Gerbera plantlets 1.2.3 Disinfection good training cut explants were inoculated into the differentiation medium. Induced 4O ~ 125d, emerged. Transfer proliferation, 15 ~ 20d subculture 1. The 15 ~ 20d can be hardened rooting out the bottles, after the heel 45d colonization. 2 Results 2.1 Induction of different diameter of the bud emergence get vaccinated to differentiation medium, induction training 4O ~ 125d after emergence. Different varieties, induced emergence time is different. Found by comparing the size on the induction of bud sprouting rate greatly. From Table 1, the outer diameter of 0.6 ~ 1.0cm block of explants induced the highest rate of budding; followed by 1.1 ~ 1.5cm; 1.6cm diameter above and 0.5crn less browning bud necrosis could not induce sprouting. Can be seen to bud as explants,[link widoczny dla zalogowanych], the diameter must be 0.6 ~ 1.5cm. Inoculated to different hormone levels and types of explants in the medium can form a callus, but callus browning occurs further developed necrosis, no browning undifferentiated bud necrosis, bud differentiation 3 situation. Table 1 Induction of different diameter bud emergence condition in Table 2 induced sprouting in different media situation in Canada with high concentrations of BA, BA with high concentrations of mixed KT MS medium induced germination is better. Reduce the concentration of BA decreased budding rate, low concentration of BA produced only callus culture medium, failed to induce germination (Table 2). Calli necrosis was found that not only the diameter of the bud, and the relationship between cytokine levels also. 2.2 The proliferation and growth of seedlings ≤ accounts. Seedling growth of proliferation of the culture time, (d) a multiple port 1g3v, l, leaf color uniformity brittle ~ iggt effective seeding rate () Rong. . Rui:-R 2.3 Rooting and transplantation select robust, tidy-free rooted, inoculated to MS + IBA0.05mg / I rooting medium, cultured 15d, rooting rate of 100. The average number of roots 4 to 6, stout root, root length of 1 ~ 2cm, the optimum transplanting time. Wash attached to the culture medium, were transplanted into irrigated in perlite: peat in a 2:1 substrate to maintain humidity 9O, 1 week after fertilizer application of 0.5 solution to moisture, 30d can be shed upon the growth of normal management , 45d after planting. Plantlets were transplanted, the media has basically run out of nutrition, good facilities must be timely after transplanting Relay fertilizer. Nutrient solution instead of using fertilizer,[link widoczny dla zalogowanych], seedlings were not adversely affected the quality, cost savings. Plantlets in the bottle is very humid, the air humidity decreases after transplanting, to be ground spraying to maintain humidity for some time, and gradually reduced to the natural air humidity. -3 Summary gerbera flower stalk and buds can be used as explants for rapid propagation. The flower buds as well,[link widoczny dla zalogowanych], it has the advantages of easy to sterilization can reduce pollution induced sprouting test failures. In tissue culture and rapid propagation, the fast is the preferred target, but must pay attention to the quality of seedlings. Although some way higher multiplication factor, but the decline in the quality of seedlings, leaves slender, fragile seedlings, poor uniformity, to the vaccination, transplanting, etc. has brought many operational problems, and difficult to survive transplanting. Therefore,[link widoczny dla zalogowanych], we must consider a combination of both to achieve the rapid propagation of the best results. Gerbera plantlets heel on the temperature of requirements. 12C the following films in the low survival rate of seedlings, slow growth, heel for a long time. 12 ~ 28C higher survival rate, seedling growth is rapid, can be transplanted normal management, conservation 45d colonization. But in general, remains to be further studied heel shorten the time to reduce the output of commodity seedling regeneration time and increase productivity. Weihong High Volume Code version of any social responsibility ∞ ∞ n the Charles: News State Forest in the matter of a hot M-pass surgery ventriloquist Yang Yang Pei Pei the physical body care organizations from the group tours the small view of chrysanthemum flower plant] species irresolute Dai Chau offer parameters such as women spacious exam paper

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